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Bio-Rad mouse anti dog ige
Mouse Anti Dog Ige, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti dog ige/product/Bio-Rad
Average 92 stars, based on 8 article reviews

mouse anti dog ige - by Bioz Stars, 2026-03

92/100 stars

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Primary cilia of human thyroid epithelial cells in vitro . Confocal fluorescence micrographs of cultured human thyroid epithelial cells upon fixation with paraformaldehyde and methanol, and immunolabelling with antibodies against the cilia marker ARL13B (green) and counter-staining of nuclear DNA with Draq5™ (blue). Boxed areas in (A, B) are shown in higher magnification in A’ and B’, respectively. Major axis lengths of primary cilia (A, A’) are annotated in µm next to the respective cilia, demonstrating a wide length range of well-extended or bent cilia. Detected nuclei are surrounded by a red line (B, B’) . Asterisks denote apoptotic bodies and nuclei touching the image borders, which are excluded from CU Cilia detection and enumeration.

Journal: Frontiers in Endocrinology

Article Title: CU Cilia – an application for image analysis by machine learning – reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells

doi: 10.3389/fendo.2025.1588394

Figure Lengend Snippet: Primary cilia of human thyroid epithelial cells in vitro . Confocal fluorescence micrographs of cultured human thyroid epithelial cells upon fixation with paraformaldehyde and methanol, and immunolabelling with antibodies against the cilia marker ARL13B (green) and counter-staining of nuclear DNA with Draq5™ (blue). Boxed areas in (A, B) are shown in higher magnification in A’ and B’, respectively. Major axis lengths of primary cilia (A, A’) are annotated in µm next to the respective cilia, demonstrating a wide length range of well-extended or bent cilia. Detected nuclei are surrounded by a red line (B, B’) . Asterisks denote apoptotic bodies and nuclei touching the image borders, which are excluded from CU Cilia detection and enumeration.

Article Snippet: For primary cilia detection, rabbit anti-human/mouse/rat/dog ARL13B (#17711-1-AP, 1:250, Proteintech, Planegg, Germany, RRID: AB_2060867) was used, followed by secondary antibody incubation, namely, goat anti-rabbit Alexa Fluor 546 or Alexa Fluor plus 488 F(ab’) fragments (1:200, Thermo Fisher Scientific, Bremen, Germany, #A11071, RRID: AB_2534115, and #A48282TR, RRID: AB_2896346, respectively) were used.

Techniques: In Vitro, Fluorescence, Cell Culture, Marker, Staining

Detection of nuclei and primary cilia of human thyroid epithelial cells in vitro using CellProfiler™ pipelines. (A) Bar charts comparing the numbers of detected nuclei and primary cilia (a1) , cilia frequencies (a2) and cilia lengths (a3) as revealed by CellProfiler™ pipelines set up by non-expert (pink, left panels) and expert users (violet, right panels). Mean values ± standard deviations are displayed in the bar charts with individual data points indicated by circles or dots (a1–a3) . Statistical analysis was by Kruskal-Wallis multiple comparisons tests; levels of significance are indicated as **** for p<0.0001. Note that the ranges of measurements differed between non-expert and expert determinations, while the 1 h-treatments with broad-spectrum (E64d) or specific cysteine peptidase inhibitors (CA074me, Odanacatib, CathLi III) did not usually differ from controls (DMSO). (B) Merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , green in b1–b5 ; black in b1’–b5’ ) and Draq5™-stained nuclei ( B , blue in b1–b5 ) of DMSO-treated controls (b1, b1’) or Nthy-ori 3–1 cell cultures treated with broad-spectrum (b2, b2’) or specific inhibitors of cathepsin B (b3, b3’) , cathepsin K (b4, b4’) or cathepsin L (b5, b5’) , respectively. Note that corresponding single channels of anti-ARL13B-positive primary cilia are shown in inverted contrast (b1’–b5’) for clarity. Scale bars represent 50 µm. Identical images were analyzed with the two different pipelines with n=9 technical replicates, except n=8 for Odanacatib-treated cells.

Journal: Frontiers in Endocrinology

Article Title: CU Cilia – an application for image analysis by machine learning – reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells

doi: 10.3389/fendo.2025.1588394

Figure Lengend Snippet: Detection of nuclei and primary cilia of human thyroid epithelial cells in vitro using CellProfiler™ pipelines. (A) Bar charts comparing the numbers of detected nuclei and primary cilia (a1) , cilia frequencies (a2) and cilia lengths (a3) as revealed by CellProfiler™ pipelines set up by non-expert (pink, left panels) and expert users (violet, right panels). Mean values ± standard deviations are displayed in the bar charts with individual data points indicated by circles or dots (a1–a3) . Statistical analysis was by Kruskal-Wallis multiple comparisons tests; levels of significance are indicated as **** for p<0.0001. Note that the ranges of measurements differed between non-expert and expert determinations, while the 1 h-treatments with broad-spectrum (E64d) or specific cysteine peptidase inhibitors (CA074me, Odanacatib, CathLi III) did not usually differ from controls (DMSO). (B) Merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , green in b1–b5 ; black in b1’–b5’ ) and Draq5™-stained nuclei ( B , blue in b1–b5 ) of DMSO-treated controls (b1, b1’) or Nthy-ori 3–1 cell cultures treated with broad-spectrum (b2, b2’) or specific inhibitors of cathepsin B (b3, b3’) , cathepsin K (b4, b4’) or cathepsin L (b5, b5’) , respectively. Note that corresponding single channels of anti-ARL13B-positive primary cilia are shown in inverted contrast (b1’–b5’) for clarity. Scale bars represent 50 µm. Identical images were analyzed with the two different pipelines with n=9 technical replicates, except n=8 for Odanacatib-treated cells.

Techniques: In Vitro, Staining

Cysteine peptidase activity at primary cilia of human thyroid epithelial cells in vitro . Confocal fluorescence micrographs of cultured human thyroid epithelial cells upon incubation with the cysteine peptidase-specific activity-based probe DCG-04 for 1 h ( A , green), fixation with paraformaldehyde and methanol, and immunostaining with antibodies against the cilia marker ARL13B ( A , red; B-D , green). Non-treated controls were immuno-stained with antibodies specific for ARL13B ( B-D , green) and cathepsin B ( B , red), cathepsin K ( C , red) or cathepsin L ( D , red). Counter-staining of nuclear DNA was with Draq5™ (a1, a5, b1, b5, c1, c5, d1, d5, blue; N, nuclei). For better contrast, the single fluorescence channels are displayed as grey scale images with inverted colors, while merged channel images are displayed as indicated. Arrowheads denote vesicular cathepsin staining in the peri-nuclear regions, whereas circles indicate immuno-recognized puncta in the extracellular space. Boxed areas in a1-a4, b1-b4, c1-c4, and d1-d4 are shown in higher magnification in a5-a8, b5-b8, c5-c8, and d5-d8, respectively. Note that some primary cilia of human thyroid epithelial cells in vitro are positive for cysteine peptidase activity (cyan boxed cilia in A ), while others lacked proteolytic activity (red boxed cilia in A ). Cathepsins B and L are abundantly detected in endo-lysosomal vesicles ( B, D , arrowheads) that are typically located around the base of primary cilia which originate from the centrosomes of the microtubule-organizing centers in juxta-nuclear position. Cathepsin K is more abundant throughout the cytoplasm and present in the extracellular space ( C , circles). Scale bars represent 20 µm in a1-a4, b1-b4, c1-c4 and d1-d4, while 5 µm are indicated in a5-a8, b5-b8, c5-c8, and d5-d8, respectively.

Journal: Frontiers in Endocrinology

Article Title: CU Cilia – an application for image analysis by machine learning – reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells

doi: 10.3389/fendo.2025.1588394

Figure Lengend Snippet: Cysteine peptidase activity at primary cilia of human thyroid epithelial cells in vitro . Confocal fluorescence micrographs of cultured human thyroid epithelial cells upon incubation with the cysteine peptidase-specific activity-based probe DCG-04 for 1 h ( A , green), fixation with paraformaldehyde and methanol, and immunostaining with antibodies against the cilia marker ARL13B ( A , red; B-D , green). Non-treated controls were immuno-stained with antibodies specific for ARL13B ( B-D , green) and cathepsin B ( B , red), cathepsin K ( C , red) or cathepsin L ( D , red). Counter-staining of nuclear DNA was with Draq5™ (a1, a5, b1, b5, c1, c5, d1, d5, blue; N, nuclei). For better contrast, the single fluorescence channels are displayed as grey scale images with inverted colors, while merged channel images are displayed as indicated. Arrowheads denote vesicular cathepsin staining in the peri-nuclear regions, whereas circles indicate immuno-recognized puncta in the extracellular space. Boxed areas in a1-a4, b1-b4, c1-c4, and d1-d4 are shown in higher magnification in a5-a8, b5-b8, c5-c8, and d5-d8, respectively. Note that some primary cilia of human thyroid epithelial cells in vitro are positive for cysteine peptidase activity (cyan boxed cilia in A ), while others lacked proteolytic activity (red boxed cilia in A ). Cathepsins B and L are abundantly detected in endo-lysosomal vesicles ( B, D , arrowheads) that are typically located around the base of primary cilia which originate from the centrosomes of the microtubule-organizing centers in juxta-nuclear position. Cathepsin K is more abundant throughout the cytoplasm and present in the extracellular space ( C , circles). Scale bars represent 20 µm in a1-a4, b1-b4, c1-c4 and d1-d4, while 5 µm are indicated in a5-a8, b5-b8, c5-c8, and d5-d8, respectively.

Techniques: Activity Assay, In Vitro, Fluorescence, Cell Culture, Incubation, Immunostaining, Marker, Staining

Elongation of primary cilia extending from human thyroid epithelial cells upon treatment with cysteine peptidase inhibitors for 24 h as revealed by CellProfiler™ and CU Cilia analyses. (A) Bar charts comparing the numbers of detected nuclei and primary cilia (a1) , cilia frequencies (a2) and cilia lengths (a3) as revealed by CellProfiler™ pipelines set up by an expert user (violet, left panels) and CU Cilia (green, right panels). Identical images (n=9) were analyzed with both approaches. Mean values ± standard deviations are displayed in the bar charts with individual data indicated by circles or dots (a1–a3) . Note that both approaches yielded comparable results. Cilia elongation was observed upon treatment with E46d and Odanacatib for 24 h (a3) . Statistical analysis was by Kruskal-Wallis and Dunn’s multiple comparisons tests; levels of significance are indicated as * for p<0.01, ** for p<0.001, and **** for p<0.0001. (B) Merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , green in b1–b5 ; black in b1’–b5’ ) and Draq5™-stained nuclei ( B , blue in b1–b5 ) of DMSO-treated controls (b1, b1’) or Nthy-ori 3–1 cell cultures treated with broad-spectrum (b2, b2’) or specific inhibitors of cathepsin B (b3, b3’) , cathepsin K (b4, b4’) or cathepsin L (b5, b5’) , respectively. Note that corresponding single channels of anti-ARL13B-positive primary cilia are shown in inverted contrast (b1’–b5’) for clarity. Scale bars represent 50 µm. Cathepsin L inhibitor III treatment for 24 h was cytotoxic towards Nthy-ori 3–1 cells as obvious from fewer detected nuclei (a1) and more abundant apoptotic bodies (b5) .

Journal: Frontiers in Endocrinology

Article Title: CU Cilia – an application for image analysis by machine learning – reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells

doi: 10.3389/fendo.2025.1588394

Figure Lengend Snippet: Elongation of primary cilia extending from human thyroid epithelial cells upon treatment with cysteine peptidase inhibitors for 24 h as revealed by CellProfiler™ and CU Cilia analyses. (A) Bar charts comparing the numbers of detected nuclei and primary cilia (a1) , cilia frequencies (a2) and cilia lengths (a3) as revealed by CellProfiler™ pipelines set up by an expert user (violet, left panels) and CU Cilia (green, right panels). Identical images (n=9) were analyzed with both approaches. Mean values ± standard deviations are displayed in the bar charts with individual data indicated by circles or dots (a1–a3) . Note that both approaches yielded comparable results. Cilia elongation was observed upon treatment with E46d and Odanacatib for 24 h (a3) . Statistical analysis was by Kruskal-Wallis and Dunn’s multiple comparisons tests; levels of significance are indicated as * for p<0.01, ** for p<0.001, and **** for p<0.0001. (B) Merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , green in b1–b5 ; black in b1’–b5’ ) and Draq5™-stained nuclei ( B , blue in b1–b5 ) of DMSO-treated controls (b1, b1’) or Nthy-ori 3–1 cell cultures treated with broad-spectrum (b2, b2’) or specific inhibitors of cathepsin B (b3, b3’) , cathepsin K (b4, b4’) or cathepsin L (b5, b5’) , respectively. Note that corresponding single channels of anti-ARL13B-positive primary cilia are shown in inverted contrast (b1’–b5’) for clarity. Scale bars represent 50 µm. Cathepsin L inhibitor III treatment for 24 h was cytotoxic towards Nthy-ori 3–1 cells as obvious from fewer detected nuclei (a1) and more abundant apoptotic bodies (b5) .

Techniques: Staining

Elongation and thinning of primary cilia extending from human thyroid epithelial cells upon treatment with general cysteine peptidase and cathepsin K-specific inhibitors for 24 h as revealed by advanced CU Cilia analyses. (A) Bar charts and box plots comparing the areas of primary cilia (a1) , major axis length (a2) , minor axis length (a3) , cilia perimeters (a4) , cilia skeleton lengths (a5) , mean distances of cilia to nearest center of mass (a6) or to nuclei boundaries (a7) as well as form factor (a8) or eccentricity of primary cilia (a9) as revealed by CU Cilia. Images (n=6) of DMSO-treated controls or Nthy-ori 3–1 cell cultures treated for 24 h with broad-spectrum or specific inhibitors of cathepsin B, cathepsin K, or cathepsin L, respectively, were analyzed and data is denoted as indicated. Mean values ± standard deviations are displayed in the bar charts with individual data indicated by circles or dots (a1–a5, a8–a9) , while box plots are displayed in a6 and a7 . Statistical analysis was conducted by Kruskal-Wallis and Dunn’s multiple comparisons tests; levels of significance are indicated as ** for p<0.01, *** for p<0.001, and **** for p<0.0001. Cilia elongation (a2, a4, a5) and cilia thinning (a3) was observed upon treatment with CA074me, E46d and Odanacatib for 24 h, while more elliptic cilia were observed in E64d- and Odanacatib-treated Nthy-ori 3–1 cells, only (a8, a9) . (B) Output images generated by CU Cilia consisting of merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , white) and Draq5™-stained nuclei ( B , blue) with identified objects boxed ( B , orange) and object sizes indicated in white font (B) of Nthy-ori 3–1 control cell cultures. Schematic drawing summarizing the advanced features of CU Cilia measurements.

Journal: Frontiers in Endocrinology

Article Title: CU Cilia – an application for image analysis by machine learning – reveals significance of cysteine cathepsin K activity for primary cilia of human thyroid epithelial cells

doi: 10.3389/fendo.2025.1588394

Figure Lengend Snippet: Elongation and thinning of primary cilia extending from human thyroid epithelial cells upon treatment with general cysteine peptidase and cathepsin K-specific inhibitors for 24 h as revealed by advanced CU Cilia analyses. (A) Bar charts and box plots comparing the areas of primary cilia (a1) , major axis length (a2) , minor axis length (a3) , cilia perimeters (a4) , cilia skeleton lengths (a5) , mean distances of cilia to nearest center of mass (a6) or to nuclei boundaries (a7) as well as form factor (a8) or eccentricity of primary cilia (a9) as revealed by CU Cilia. Images (n=6) of DMSO-treated controls or Nthy-ori 3–1 cell cultures treated for 24 h with broad-spectrum or specific inhibitors of cathepsin B, cathepsin K, or cathepsin L, respectively, were analyzed and data is denoted as indicated. Mean values ± standard deviations are displayed in the bar charts with individual data indicated by circles or dots (a1–a5, a8–a9) , while box plots are displayed in a6 and a7 . Statistical analysis was conducted by Kruskal-Wallis and Dunn’s multiple comparisons tests; levels of significance are indicated as ** for p<0.01, *** for p<0.001, and **** for p<0.0001. Cilia elongation (a2, a4, a5) and cilia thinning (a3) was observed upon treatment with CA074me, E46d and Odanacatib for 24 h, while more elliptic cilia were observed in E64d- and Odanacatib-treated Nthy-ori 3–1 cells, only (a8, a9) . (B) Output images generated by CU Cilia consisting of merged channel confocal laser scanning micrographs depicting ARL13B-positive primary cilia ( B , white) and Draq5™-stained nuclei ( B , blue) with identified objects boxed ( B , orange) and object sizes indicated in white font (B) of Nthy-ori 3–1 control cell cultures. Schematic drawing summarizing the advanced features of CU Cilia measurements.

Techniques: Generated, Staining, Control

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